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Extraction of Macrolide Antibiotics from Fermentation Broth Using Triple Countercurrent Centrifugal Extraction Process

Tylosin extracted from fermentation broth is a macrolide antibiotic. At present, the domestic conventional extraction process is generally - after pretreatment of the tylosin fermentation broth, the fermentation filtrate is obtained by filtration, and then the fermentation filtrate is extracted with ethyl acetate to obtain an organic phase rich in tylosin, and then acid water Back-extraction, neutralization, decolorization, concentration and drying to obtain the fine product of tylosin tartrate or tylosin phosphate.

In industrial production, enterprises generally need to improve the above ordinary extraction and purification processes. Trichloromethane or dichloromethane can be used to extract tylosin from the fermentation filtrate. The extraction rate of impurities is extremely high, and a large amount of red pigment and other substances can be extracted. Impurities, however, it is difficult to remove some impurities in the later stage, which will seriously affect the quality and yield of tylosin.

According to the person in charge of Zhengzhou TIEI Extraction Application Center, for this problem, the technology of combining compound extractant and WL-M three-stage countercurrent centrifugal extraction can be used to optimize the purification process of tylosin and effectively improve the production of tylosin. quality and yield. Among them, the compound extractant is composed of ethyl acetate-chloroform (or trichloroethane)-petroleum ether. After three-stage countercurrent extraction by CWL-M centrifugal extractor, the purity and quality of tylosin products are greatly improved. The total content of tylosin in the extract is not less than 98%.

At the same time, the CWL-M series three-stage countercurrent centrifugal extraction process is adopted, which improves the extraction yield and shortens the extraction cycle, greatly improving production efficiency. Its host equipment, CWL-M series centrifugal extractor, has a simple and stable structure, excellent mixing and separation effects, and can realize automatic control. The material of the equipment can be selected from perfluorinated materials, which can resist strong acid corrosion.